ABSTRACT
ObjectiveTo investigate the effect of improvement in internal environment after plasma exchange on the bioactivity of peripheral blood CD34+ cells in patients with acute-on-chronic (subacute) liver failure. MethodsThe peripheral blood CD34+ cells from patients with acute-on-chronic (subacute) liver failure were cultured in vitro using the medium containing with the serum collected before or after plasma exchange. The growth curves of the two groups of cells were recorded and compared. RT-PCR was used to detect the expression of the cell surface markers PK-M2, Integrin-β1, and L-PK, and immunofluorescence was used to detect the expression of Integrin-β1. A repeated measures analysis of variance was used for comparison of continuous data between groups. ResultsThe CD34+ cells in the medium containing the serum collected after plasma exchange grew better than those in the medium containing the serum collected before plasma exchange (P＜0.05). PK-M2 and Integrin-β1 were detected in the CD34+ cells of both groups, but L-PK was not detected in either group. ConclusionFor patients with liver failure, the improvement in internal environment after plasma exchange helps to maintain the bioactivity of peripheral blood CD34+ cells, thus improving the efficacy of stem cell transplantation for liver failure.
ABSTRACT
As a member of regenerating islet-derived family,regenerating islet-derived type Ⅳ(RegⅣ)is involved in cell proliferation and differentiation. RegⅣ is closely related to the occurrence and develop-ment of tumor,tumor cell proliferation and invasion and anti-apoptosis,and it may be a potential molecular tar-get of targeting therapy in cancer.